We are pleased to share the outcome of a collaboration between VitroScreen and the University of Milan (Department of Pharmaceutical Sciences) that resulted in the publication of a scientific article in the International Journal of Molecular Sciences.
VitroScreen ORA® dermis model was adopted as advanced biological system to investigate the mechanism of action of L-carnosine by a proteomic approach based on high-resolution spectrometry analysis generated by Orbitrap Fusion Tribrid Mass Spectrometer.
The high predictive value of the proteomic approach together with the high biological relevance of VitroScreen ORA® models provide a fit-for-purpose tool for preclinical studies and personalized therapies development.
VitroScreen ORA® dermis model is a scaffold-free spheroids produced by self assembly of primary dermal fibroblasts isolated from donors aged 50 years cultivated during 7 and 14 days.
The results have underlined an interesting and significant modulation of the following proteomic profiles:
- Increase of ECM adhesion key proteins during the time
- Increase of structural and mechanical proprieties and elastic features
- Progressive remodelling of ECM and elastic components
- Cytoskeleton re-organization
- Activation of mitochondrial activity
- Increase of cellular metabolism
- Apoptosis