We are pleased to share the outcome of a collaboration between VitroScreen and the University of Milan (Department of Pharmaceutical Sciences) that resulted in the publication of a scientific article in the International Journal of Molecular Sciences.

VitroScreen ORA® dermis model was adopted as advanced  biological system  to investigate the mechanism of action of L-carnosine by a proteomic approach based on high-resolution spectrometry analysis generated by Orbitrap Fusion Tribrid Mass Spectrometer.

The high predictive value of the proteomic approach together with the high biological relevance of VitroScreen ORA® models provide a fit-for-purpose tool for preclinical studies and personalized therapies development.

VitroScreen ORA® dermis model is a scaffold-free spheroids produced by self assembly of primary dermal fibroblasts isolated from donors aged 50 years cultivated during 7 and 14 days.

The results have underlined an interesting and significant modulation of the following proteomic profiles:

  • Increase of ECM adhesion key proteins during the time
  • Increase of structural and mechanical proprieties and elastic features
  • Progressive remodelling of ECM and elastic components
  • Cytoskeleton re-organization
  • Activation of mitochondrial activity
  • Increase of cellular metabolism
  • Apoptosis
Read the full article